报告摘要：
组织工程和再生医学（TERM）策略取决于合适的材料支撑，保持或封装细胞。在众多方法中，使用包括各种海洋原材料在内的天然聚合物是再生不同类型组织的最佳选择。除了选择合适的材料体系外，有效的合成方法可以得到优良的基质支架，许多情况下会加入生物活性剂或分化剂。此外，还应该选择合适的细胞系，我们一直在使用不同的人源细胞，比如来自骨髓的间充质干细胞，来自人脂肪组织的间充质干细胞，来自人羊水、羊膜细胞以及人脐带细胞。发展细胞培养的动态方法；利用不同的方法刺激细胞在3D环境中的分化；以及利用纳米系统诱导其分化和细胞内化这些都是技术发展的关键部分，也是我们的研究团队致力开发的部分。本次报告我们将讨论用于新型再生疗法的每一种复合材料和细胞的开发潜力，描述不同细胞的使用情况及其与不同天然来源的可降解支架和智能水凝胶的相互作用，介绍TERM策略中关于再生不同类型组织的若干实例，介绍使用原始的高通量方法来观察材料和细胞的相互作用情况。
报告人简介:
Rui L. Reis 美国工程院外籍院士，葡萄牙米尼奥大学教授、副校长，长期致力于生物医用材料、组织工程、再生医学和造血干细胞方面的基础和应用研究，创立了3B生物实验中心、欧洲组织工程与再生医学研究所，是Journal of Tissue Engineering and Regenerative Medicine杂志主编。已发表论文1500多篇，获30专利多项。Rui L. Reis 领导的3B生物实验中心是欧洲顶尖实验室，致力于生物技术、生物学、生物医学工程和材料科学的结合。主要研究领域包括新材料开发、药物传递、组织工程、再生医学、纳米医学、干细胞分离与分化等。
Rui L. Reis 1,2,3
13B's Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; 2ICVS/3B’s–PT Government Associate Laboratory, Braga/Guimarães, Portugal; 3The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, 4805-017 Barco, Guimarães, Portugal; rgreis@i3bs.uminho.pt
The selection of a proper material to be used as a scaffold or as a hydrogel to support, hold or encapsulate cells is both a critical and a difficult choice that will determine the success of failure of any tissue engineering and regenerative medicine (TERM) strategy.
We believe that the use of natural origin polymers, including a wide range of marine origin materials, is the best option for many different approaches that allow for the regeneration of different tissues. In addition to the selection of appropriate material systems it is of outmost importance the development of processing methodologies that allow for the production of adequate scaffolds/matrices, in many cases incorporating bioactive/differentiation agents in their structures.
Furthermore an adequate cell source should be selected. In many cases efficient cell isolation, expansion and differentiation, and in many cases the selection of a specific sub-population, methodologies should be developed and optimized. We have been using different human cell sources namely: mesenchymal stem cells from bone marrow, mesenchymal stem cells from human adipose tissue, human cells from amniotic fluids and membranes and cells obtained from human umbilical cords.
The development of dynamic ways to culture the cells and of distinct ways to stimulate their differentiation in 3D environments, as well as the use of nano-based systems to induce their differentiation and internalization into cells, is also a key part of some of the strategies that are being developed in our research group.
The potential of each combination materials/cells, to be used to develop novel useful regeneration therapies will be discussed. The use of different cells and their interactions with different natural origin degradable scaffolds and smart hydrogels will be described. Several examples of TERM strategies to regenerate different types of tissues will be presented. This will include the use of original high-throughput methodologies to look at materials/cell interactions.
